The successful treatment of Diphyllobothrium latum, Taenia saginata and Diplogonopolus grandis infections with Gastrografin is herein reported. Eleven subjects (8 cases of D. latum, 2 of T. saginata and one of D. grandis infection) found to have tapeworm infection based on the history of discharge of gravid segments and/or positivity for tapeworm egg were first pretreated with barium enema procedure and were treated with 300 to 500 ml Gastrografin by duodenal manipulation. All cases who indicated the presence of the tapeworm as a radiolucent shadows were completely treated with Gastrografin. All tapeworms with scolex expelled were actively moving in the saline. The time of treatment (from 15 to 30 min) was shorter than that of other medical treatments. No side effects were observed. In the present study, the treatment with Gastrografin was found to be a satisfactory anthelmintic method against D. latum, T. saginata and D. grandis infection.