Effect of Gaseous Atmosphere on Different Supplementation Medium for In Vitro Canine Oocyte Maturation

Abstract

The aim of the present research was to verify the influence of two different atmosphere gases (A: 5%CO2, B: 5%CO2, 5%O2 and 90%N2) on three different maturation media on in vitro maturation (IVM) rates for oocytes from anoestrous and diestrous bitches that were cultured in vitro for 48h. Oocytes harvested from bitches were selected and allocated into three maturation media groups, cultured in atmosphere A or B. Group 1, oestrous bitch serum; Group 2, bovine serum albumin (BSA), glutamine, cisteamine; Group 3, BSA, estrogen (E2), epidermal growth factor (EGF). Progression of nuclear maturation was evaluated under fluorescent microscopy after staining with Hoechst 33342

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Cite this paper

@inproceedings{Gonzlez2014EffectOG, title={Effect of Gaseous Atmosphere on Different Supplementation Medium for In Vitro Canine Oocyte Maturation}, author={Noelia Gonz{\'a}lez and Lydia Gil and Glenda L. R{\'i}os and Jesica R. Canizo and Jos{\'e} Ignacio De Blas and Ricardo Horacio Alberio}, year={2014} }