Effect of EDTA and citrate on the functional activity of the first component of complement, C1, and the C1q subcomponent.

@article{Zapf1985EffectOE,
  title={Effect of EDTA and citrate on the functional activity of the first component of complement, C1, and the C1q subcomponent.},
  author={Silke Zapf and M. R. de Loos},
  journal={Immunobiology},
  year={1985},
  volume={170 3},
  pages={123-32}
}
The first component of complement, C1, is a calcium-dependent complex of the three distinct subcomponents, C1q, C1r, and C1s. Earlier observations revealed that treatment of C1 with EDTA led to a loss of hemolytic C1 activity even after recalcification. Therefore, it was of interest to study whether EDTA has an additional effect on C1 and its subcomponents, beside its chelating capacity. The chelating effect of EDTA was compared to that of citrate. It was found that treatment of C1 or C1 with… CONTINUE READING