Effect of Docosahexaenoic Acid on Voltage-Independent Ca2+ Entry Pathways in Cultured Vascular Smooth Muscle Cells Stimulated with 5-Hydroxytryptamine.

  title={Effect of Docosahexaenoic Acid on Voltage-Independent Ca2+ Entry Pathways in Cultured Vascular Smooth Muscle Cells Stimulated with 5-Hydroxytryptamine.},
  author={Takuji Machida and Akina Onoguchi and Kenji Iizuka and Sayuri Ishibashi and Mikiko Yutani and Masahiko Hirafuji},
  journal={Biological \& pharmaceutical bulletin},
  volume={40 6},
We previously reported that docosahexaenoic acid (DHA) inhibits an increase in intracellular Ca2+ concentration ([Ca2+]i) in cultured rat vascular smooth muscle cells (VSMCs) through a mechanism involving mainly voltage-dependent Ca2+ channels; however, the effect of DHA on voltage-independent pathways, such as store-operated and receptor-operated Ca2+ entry, and Ca2+ entry through Na+/Ca2+ exchanger (NCX), has not been clarified. In the present study, we investigated the effect of DHA… Expand
2 Citations
Docosahexaenoic acid suppresses angiotensin II-induced A7r5 vascular smooth muscle cell proliferation and migration under pulsatile pressure stress.
Pulsatile pressure stress and DHA inhibited angiotensin II-induced VSMC proliferation and migration under abnormal pressure conditions, which may contribute to the understanding of the beneficial effect of DHA on various cardiovascular disorders. Expand
Inhibition of RhoA/ROCK signaling pathway ameliorates hypoxic pulmonary hypertension via HIF‐1&agr;‐dependent functional TRPC channels
Results indicate that inhibition of the RhoA/ROCK signaling pathway ameliorates HPH via HIF‐1&agr;‐dependent functional TRPCs, and down‐regulates the expressions and functions of TRPC channels. Expand


Inhibition by docosahexaenoic acid of receptor-mediated Ca(2+) influx in rat vascular smooth muscle cells stimulated with 5-hydroxytryptamine.
The results suggest that the specific inhibition of intracellular Ca(2+) dynamics in vascular smooth muscle cells may contribute to the beneficial properties of docosahexaenoic acid on cardiovascular disorders. Expand
Effects of docosahexaenoic acid on large-conductance Ca2+-activated K+ channels and voltage-dependent K+ channels in rat coronary artery smooth muscle cells
Findings indicate that the vasorelaxation effects of DHA on vascular smooth muscle cells are mainly due to its activation of BKCa channels, which can activate BK Ca channels and block KV channels in rat CASMCs. Expand
Effect of 5-hydroxytryptamine on intracellular calcium dynamics in cultured rat vascular smooth muscle cells.
The present study elucidated the precise mechanism of 5-hydroxytryptamine (5-HT)-induced increase of intracellular Ca2+ concentration ([Ca2+]i) in cultured vascular smooth muscle cells isolated from rat aortic media, and suggested that PTX-sensitive G protein and cyclic AMP seem to be not involved. Expand
Agonist-evoked calcium entry in vascular smooth muscle cells requires IP3 receptor-mediated activation of TRPC1.
Results indicate that in aortic smooth muscle cells, TRPC1 is not only involved in Ca2- entry activated by store depletion but also in receptor-operated Ca2+ entry, which requires inositol (1,4,5) triphosphate receptor activation. Expand
Regulation of intracellular calcium levels by polyunsaturated fatty acids, arachidonic acid and docosahexaenoic acid, in astrocytes: possible involvement of phospholipase A2.
It is suggested that prolonged exposure of astrocytes to AA and DHA brought the cells to a new steady state of a moderately elevated [Ca2+]i level, where the cells became virtually insensitive to external stimuli, which can be considered as a mechanism of self-protection. Expand
Orai1 and TRPC1 Proteins Co-localize with CaV1.2 Channels to Form a Signal Complex in Vascular Smooth Muscle Cells*
It is found that serotonin (5-HT) or store depletion with thapsigargin (TG) enhanced intracellular free Ca2+ concentration ([Ca2+]i) and stimulated aorta contraction, confirming that upon agonist stimulation, vessel contraction involves Ca 2+ entry due to co-activation of Orai1- and TRPC1-dependent SOCC and LTCC. Expand
TRPC1 Associates With BKCa Channel to Form a Signal Complex in Vascular Smooth Muscle Cells
The hyperpolarizing effect of TRPC1-BKCa coupling could serve to reduce agonist-induced membrane depolarization, thereby preventing excessive contraction of VSMCs to contractile agonists. Expand
Cell culture alters Ca2+ entry pathways activated by store-depletion or hypoxia in canine pulmonary arterial smooth muscle cells.
It is concluded that cell culture of canine PASMCs reorganizes IP(3) and ryanodine receptors into a common intracellular Ca(2+) compartment, and depletion of this store or hypoxia activates voltage-operated Ca( 2+) entry, reverse mode NCX, and CCE. Expand
Omega-3 fatty acids lower blood pressure by directly activating large-conductance Ca2+-dependent K+ channels
It is reported here that DHA with an EC50 of ∼500 nM rapidly and reversibly activates BK channels composed of the pore-forming Slo1 subunit and the auxiliary subunit β1, increasing currents by up to ∼20-fold, and has practical implications for the use of omega-3 fatty acids as nutraceuticals for the general public and also for the critically ill receiving omega- 3–enriched formulas. Expand
NF-κB-Dependent Upregulation of NCX1 Induced by Angiotensin II Contributes to Calcium Influx in Rat Aortic Smooth Muscle Cells.
Ang II upregulates NCX1 expression through p38 MAPK and NF-κB pathways, and reverse-mode NCX 1 plays an important part in Ang II-induced Ca2+ influx in VSMCs, which may be associated with Ang II'sinitiated store-operated channel entry. Expand