Effect of DNA sequence of Fab fragment on yield characteristics and cell growth of E. coli

@inproceedings{Kulmala2017EffectOD,
  title={Effect of DNA sequence of Fab fragment on yield characteristics and cell growth of E. coli},
  author={Antti Kulmala and Tuomas Huovinen and Urpo J Lamminm{\"a}ki},
  booktitle={Scientific Reports},
  year={2017}
}
Codon usage is one of the factors influencing recombinant protein expression. We were interested in the codon usage of an antibody Fab fragment gene exhibiting extreme toxicity in the E. coli host. The toxic synthetic human Fab gene contained domains optimized by the “one amino acid-one codon” method. We redesigned five segments of the Fab gene with a… CONTINUE READING