Effect of DNA extraction method on the apparent microbial diversity of soil.

@article{Inceolu2010EffectOD,
  title={Effect of DNA extraction method on the apparent microbial diversity of soil.},
  author={Ozg{\"u}l Inceoğlu and Eelco F Hoogwout and Patrick I. Hill and Jan Dirk van Elsas},
  journal={Applied and environmental microbiology},
  year={2010},
  volume={76 10},
  pages={3378-82}
}
Four extraction methods, including a novel one, were compared for their efficiencies in producing DNA from three contrasting agricultural soils. Molecular analyses (PCR-denaturing gradient gel electrophoresis [DGGE] and clone libraries) focusing on different microbial groups were used as assessment criteria. Per soil, the DNA yields differed between extraction methods. Clear effects of method on apparent richness and community structure were found. Actinobacterial diversity based on soil DNA… CONTINUE READING