Effect of DNA extraction and sample preservation method on rumen bacterial population.

@article{Fliegerov2014EffectOD,
  title={Effect of DNA extraction and sample preservation method on rumen bacterial population.},
  author={K. Fliegerov{\'a} and I. Tapio and A. Bonin and J. Mr{\'a}zek and M. Callegari and P. Bani and A. Bayat and J. Vilkki and J. Kope{\vc}n{\'y} and K. Shingfield and F. Boyer and E. Coissac and P. Taberlet and R. Wallace},
  journal={Anaerobe},
  year={2014},
  volume={29},
  pages={
          80-4
        }
}
The comparison of the bacterial profile of intracellular (iDNA) and extracellular DNA (eDNA) isolated from cow rumen content stored under different conditions was conducted. The influence of rumen fluid treatment (cheesecloth squeezed, centrifuged, filtered), storage temperature (RT, -80 °C) and cryoprotectants (PBS-glycerol, ethanol) on quality and quantity parameters of extracted DNA was evaluated by bacterial DGGE analysis, real-time PCR quantification and metabarcoding approach using high… Expand

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