Effect of DNA concentration on transgenesis rates in mice and pigs

@article{Nottle2001EffectOD,
  title={Effect of DNA concentration on transgenesis rates in mice and pigs},
  author={Mark. B. Nottle and K. A. Haskard and Paul John Verma and Zhong Tao Du and Christopher Gerald Grupen and Stephen M McIlfatrick and Rodney J Ashman and Sharon J. Harrison and Hilda Barlow and Peter L Wigley and Ian G Lyons and Peter J Cowan and Robert J. Crawford and P. L. Tolstoshev and Martin John Pearse and Anthony J Robins and A.J.F. d'Apice},
  journal={Transgenic Research},
  year={2001},
  volume={10},
  pages={523-531}
}
A retrospective analysis of transgenesis rates obtained in seven pronuclear microinjection programs was undertaken to determine if a relationship existed between the amount of DNA injected and transgenesis rates in the pig. Logistic regression analysis showed that as the concentration of DNA injected increased from 1 to 10 ng/μl, the number of transgenics when expressed as a proportion of the number liveborn (integration rate) increased from 4% to an average of 26%. A similar relationship was… CONTINUE READING

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