Effect of DNA binding protein Ssh12 from hyperthermophilic archaeonSulfolobus shibatae on DNA supercoiling

@article{Lou1999EffectOD,
  title={Effect of DNA binding protein Ssh12 from hyperthermophilic archaeonSulfolobus shibatae on DNA supercoiling},
  author={Huiqiang Lou and Li Ming Huang and Viet Q. Mai},
  journal={Science in China Series C: Life Sciences},
  year={1999},
  volume={42},
  pages={401-408}
}
An 11.5-ku DNA binding protein, designated as Ssh12, was purified from the hyperthermophilic archaeonSulfolobus shibatae by column chromatography in SP Sepharose, DNA cellulose and phosphocellulose. Ssh12 accounts for about 4 % of the total cellular protein. The protein is capable of binding to both negatively supercoiled and relaxed DNAs. Nick closure analysis revealed that Ssh12 constrains negative supercoils upon binding to DNA. While the ability of the protein to constrain supercoils is… CONTINUE READING

Figures and Topics from this paper.

Explore Further: Topics Discussed in This Paper

Citations

Publications citing this paper.

References

Publications referenced by this paper.
SHOWING 1-10 OF 16 REFERENCES

Structure and properties of the bacterial nucleoid, Cell

J DNA, Mol
  • J. Biol. Chem. ,
  • 1995

Small abundant DNA binding proteins from the thermoacidopbilic archaeon Su /folobu ~ shibatae constrain negative DNA supercoils

V. Q. Mai, X. Chen, R. Hong
  • J . Bacteriol .
  • 1991