Effect of DNA Extraction Methods and Sampling Techniques on the Apparent Structure of Cow and Sheep Rumen Microbial Communities

@inproceedings{Henderson2013EffectOD,
  title={Effect of DNA Extraction Methods and Sampling Techniques on the Apparent Structure of Cow and Sheep Rumen Microbial Communities},
  author={Gemma Henderson and Faith Cox and Sandra Kittelmann and Vahideh Heidarian Miri and Michael Zethof and Samantha Joan Noel and Garry Campbell Waghorn and Peter H Janssen},
  booktitle={PloS one},
  year={2013}
}
Molecular microbial ecology techniques are widely used to study the composition of the rumen microbiota and to increase understanding of the roles they play. Therefore, sampling and DNA extraction methods that result in adequate yields of microbial DNA that also accurately represents the microbial community are crucial. Fifteen different methods were used to extract DNA from cow and sheep rumen samples. The DNA yield and quality, and its suitability for downstream PCR amplifications varied… CONTINUE READING

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