Effect of Culture Medium Volume on the Development and Viability of Microinjected or Noninjected One-Cell Hybrid and CD-1 Strain Mouse Embryos


We investigated the effects of drop volume on the development of mouse presumptive zygotes to the completely hatched blastocyst stage. For this aim, we established 3 experimental groups. In the first group, we cultured hybrid presumptive zygotes to the hatched blastocyst stage in 10 presumptive zygotes per 5, 10, 20, 40 and 50 μl drops. In the second group… (More)


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