Effect of Cryopreserved Amniotic Membrane Orientation on the Expression of Limbal Mesenchymal and Epithelial Stem Cell Markers in Prolonged Limbal Explant Cultures

@article{Lunik2016EffectOC,
  title={Effect of Cryopreserved Amniotic Membrane Orientation on the Expression of Limbal Mesenchymal and Epithelial Stem Cell Markers in Prolonged Limbal Explant Cultures},
  author={Z. Lu{\vz}nik and M. Hawlina and E. Mali{\vc}ev and M. Bertolin and A. Kopitar and A. Ihan and S. Ferrari and P. Schollmayer},
  journal={PLoS ONE},
  year={2016},
  volume={11}
}
  • Z. Lužnik, M. Hawlina, +5 authors P. Schollmayer
  • Published 2016
  • Biology, Medicine
  • PLoS ONE
    • Purpose To evaluate the effect of prolonged limbal explants cultured without any scaffolds or on amniotic membrane (AM) on the viability, proliferation and differentiation potential of putative phenotypically defined cultured limbal mesenchymal (LMSC) and epithelial stem cells (LESC). Methods Limbal explants were cultivated on cryopreserved intact AM or plastic plates using medium supplemented with only human serum. AM was positioned with either the epithelial or stromal side up. The outgrowing… CONTINUE READING
    View PDF
    9 Citations
    Background Citations
    3
    Methods Citations
    2
    Results Citations
    1
    9 Citations
    Citation Type

    Citation Type

    Comparison of culture media indicates a role for autologous serum in enhancing phenotypic preservation of rabbit limbal stem cells in explant culture
    • 3
    CD200 Expression Marks a Population of Quiescent Limbal Epithelial Stem Cells with Holoclone Forming Ability
    • 6
    • PDF
    Interleukin-13 maintains the stemness of conjunctival epithelial cell cultures prepared from human limbal explants
    • 2
    • PDF
    Explant culture: An advantageous method for isolation of mesenchymal stem cells from human tissues
    • 58
    Corneal Stem Cells: Identification and Methods of Ex Vivo Expansion
    Identification and characterization of dendritic cell subtypes in preserved and cultured cadaveric human corneolimbal tissue on amniotic membrane
    • 2
    Ex vivo cultivation of limbal epithelial stem / progenitor cells : comparison of two culturing techniques
    • PDF
    Ocular surface reconstruction in limbal stem cell deficiency: current treatment options and perspectives
    Levels of oxidative DNA damage are low in ex vivo engineered human limbal epithelial tissue

    References

    SHOWING 1-10 OF 32 REFERENCES
    Ex vivo preservation and expansion of human limbal epithelial stem cells on amniotic membrane cultures
    • 265
    • PDF
    Ex vivo expanded autologous limbal epithelial cells on amniotic membrane using a culture medium with human serum as single supplement.
    • 51
    • PDF
    Effects of fibroblast origin and phenotype on the proliferative potential of limbal epithelial progenitor cells.
    • 46
    Long-Term Cultures of Human Cornea Limbal Explants Form 3D Structures Ex Vivo – Implications for Tissue Engineering and Clinical Applications
    • 19
    • PDF
    The fate of limbal epithelial progenitor cells during explant culture on intact amniotic membrane.
    • 76
    • PDF
    Gene expression profile of epithelial cells and mesenchymal cells derived from limbal explant culture
    • 31
    • PDF
    Stem cells isolated from the human stromal limbus possess immunosuppressant properties
    • 33
    • PDF
    Correlation of Cell Cycle Kinetics with p63 Expression in Human Limbal Epithelial Cells Expanded on Intact Human Amniotic Membrane
    • 14
    Characterization of corneal stromal stem cells with the potential for epithelial transdifferentiation
    • 61
    • PDF
    Comparison of intact and denuded amniotic membrane as a substrate for cell-suspension culture of human limbal epithelial cells
    • 108