Effect of Cryopreserved Amniotic Membrane Orientation on the Expression of Limbal Mesenchymal and Epithelial Stem Cell Markers in Prolonged Limbal Explant Cultures

@article{Lunik2016EffectOC,
  title={Effect of Cryopreserved Amniotic Membrane Orientation on the Expression of Limbal Mesenchymal and Epithelial Stem Cell Markers in Prolonged Limbal Explant Cultures},
  author={Z. Lu{\vz}nik and M. Hawlina and E. Mali{\vc}ev and M. Bertolin and A. Kopitar and A. Ihan and S. Ferrari and P. Schollmayer},
  journal={PLoS ONE},
  year={2016},
  volume={11}
}
  • Z. Lužnik, M. Hawlina, +5 authors P. Schollmayer
  • Published 2016
  • Biology, Medicine
  • PLoS ONE
  • Purpose To evaluate the effect of prolonged limbal explants cultured without any scaffolds or on amniotic membrane (AM) on the viability, proliferation and differentiation potential of putative phenotypically defined cultured limbal mesenchymal (LMSC) and epithelial stem cells (LESC). Methods Limbal explants were cultivated on cryopreserved intact AM or plastic plates using medium supplemented with only human serum. AM was positioned with either the epithelial or stromal side up. The outgrowing… CONTINUE READING
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