Effect of Cryoconservation on Seed Germination of Rare Tropical Orchids

@article{Nikishina2004EffectOC,
  title={Effect of Cryoconservation on Seed Germination of Rare Tropical Orchids},
  author={T. V. Nikishina and A. S. Popov and Galina L Kolomeitseva and B. N. Golovkin},
  journal={Russian Journal of Plant Physiology},
  year={2004},
  volume={48},
  pages={810-815}
}
In search of a procedure for cryoconservation, seeds of five species of tropical orchids and of one intergeneric hybrid were germinatedin vitroas depended on illumination. The other portion of seeds was frozen in liquid nitrogen (–196°C) and germinated after thawing. The viability of seeds, the percentage and rate of germination, as well as the growth rate of the developed protocorms were evaluated. At early stages of development, light inhibited growth in most species examined. The storage of… 
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Germination rates of seeds after their exposure to liquid nitrogen were species-depended and could be either higher or lower than in the unfrozen control, while growth rates of protocorms of the same species obtained from seeds collected in various Russia regions and cultured for 5 months were no significant difference.
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A preliminary assessment of genetic diversity at the population level provides for the maximum possible mobilization of the species gene pool in the Far Eastern flora.
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This study improves, an efficient protocol was established that maintained seed viability and enhanced the germination rates of seeds, compared with previously described cryopreservation methods, and the germinated seeds showed normal morphology of both vegetative and reproductive organs.
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