Effect of C-domain N-glycosylation and deletion on rat pancreatic alpha-amylase secretion and activity.

@article{Doyon2002EffectOC,
  title={Effect of C-domain N-glycosylation and deletion on rat pancreatic alpha-amylase secretion and activity.},
  author={Yannick Doyon and William C. Home and Philippe Daull and Denis Lebel},
  journal={The Biochemical journal},
  year={2002},
  volume={362 Pt 1},
  pages={259-64}
}
Even though all animal alpha-amylases include glycosylation sequons (Asn-Xaa-Thr/Ser) in their sequences, amylases purified from natural sources are not quantitatively glycosylated. When wild-type rat pancreatic alpha-amylase, which contains two glycosylation sequons, was expressed in animal cell lines the protein displayed a very low rate of glycosylation (approx. 2%), even after Brefeldin A treatment to increase the contact with the glycosylation machinery. Site-directed mutagenesis of the… CONTINUE READING

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