Effect of C-Terminal of Human Cytosolic Thymidine Kinase (TK1) on in Vitro Stability and Enzymatic Properties

@article{Zhu2006EffectOC,
  title={Effect of C-Terminal of Human Cytosolic Thymidine Kinase (TK1) on in Vitro Stability and Enzymatic Properties},
  author={C. Zhu and L. S. Harlow and Dvora Berenstein and Sten Munch-Petersen and Birgitte Munch‐Petersen},
  journal={Nucleosides, Nucleotides \& Nucleic Acids},
  year={2006},
  volume={25},
  pages={1185 - 1188}
}
Thymidine kinase (TK1) is a key enzyme in the salvage pathway of nucleotide metabolism and catalyzes the first rate-limiting step in the synthesis of dTTP, transfer of a gamma-phosphate group from a nucleoside triphosphate to the 5′-hydroxyl group of thymidine, thus forming dTMP. TK1 is cytosolic and its activity fluctuates during cell cycle coinciding with the DNA synthesis rate and disappears during mitosis. This fluctuation is important for providing a balanced supply of dTTP for DNA… 

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TLDR
It is demonstrated that residues near the carboxyl terminus of TK are essential for the cell cycle phase-specific degradation of the enzyme.

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TLDR
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  • Chemistry, Biology
    Proceedings of the National Academy of Sciences of the United States of America
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TLDR
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TLDR
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TLDR
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