Effect of Bordetella pertussis toxin on ADP-ribosylation of membrane proteins, adenylate cyclase activity and insulin release in rat pancreatic islets.

@article{Malaisse1984EffectOB,
  title={Effect of Bordetella pertussis toxin on ADP-ribosylation of membrane proteins, adenylate cyclase activity and insulin release in rat pancreatic islets.},
  author={Willy Jean Malaisse and Michal Svoboda and S. P. Dufrane and Francine Malaisse-Lagae and Jean Christophe},
  journal={Biochemical and biophysical research communications},
  year={1984},
  volume={124 1},
  pages={190-6}
}
Exposure of rat pancreatic islet membranes to [alpha-32P]-NAD+ in the presence of Bordetella Pertussis toxin (islet-activating protein) reveals the ADP-ribosylation of a peptide with a Mr close to 41 kDa, which corresponds to the alpha-subunit of the guanine nucleotide regulatory protein Ni. Islets removed from rats pretreated with the Bordetella Pertussis toxin display a specific increase in adenylate cyclase responsiveness to GTP and are characterized by a resistance to the inhibitory action… CONTINUE READING