Effect of Bacillus subtilis spo0A mutation on cell wall lytic enzymes and extracellular proteases, and prevention of cell lysis.

@article{Kodama2007EffectOB,
  title={Effect of Bacillus subtilis spo0A mutation on cell wall lytic enzymes and extracellular proteases, and prevention of cell lysis.},
  author={Takeko Kodama and Keiji Endo and Katsutoshi Ara and Katsuya Ozaki and Hiroshi Kakeshita and Kunio Yamane and Junichi Sekiguchi},
  journal={Journal of bioscience and bioengineering},
  year={2007},
  volume={103 1},
  pages={13-21}
}
The Bacillus subtilis spo0A mutant is an adequate host for extracellular protein production (e.g., alpha-amylase). However the mutant was prone to cell lysis. SDS-PAGE and zymography of cell wall lytic proteins indicated that the spo0A mutant contained high amounts of two major autolysins (LytC [CwlB] and LytD [CwlG]) and two minor cell wall lytic enzymes (LytE [CwlF] and LytF [CwlE]). On the other hand, the expression of eight extracellular protease genes was very poor or absent in the spo0A… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 13 extracted citations

Microbial Production

Springer Japan • 2014
View 1 Excerpt

Similar Papers

Loading similar papers…