Effect of Antipeptide Antibodies Directed against Three Domains of Connexin43 on the Gap Junctional Permeability of Cultured Heart Cells

  title={Effect of Antipeptide Antibodies Directed against Three Domains of Connexin43 on the Gap Junctional Permeability of Cultured Heart Cells},
  author={Bruno Bastide and Th{\'e}r{\`e}se Jarry-Guichard and Jean Paul Briand and Jean D{\'e}l{\`e}ze and Daniel B. Gros},
  journal={The Journal of Membrane Biology},
Abstract. Cell-to-cell communication can be blocked by intracellular injections of antibodies raised against gap junction proteins, but the mechanism of channel obstruction is unknown. Binding to connexins could lead to a conformational change, interfere with regulatory domains or cause a steric hindrance. To address these questions, the effects on cell-to-cell communication of affinity purified polyclonal antibodies raised against peptides reproducing the intracellular sequences 5–17, 314–322… 

LacSwitch II regulation of connexin43 cDNA expression enables gap-junction single-channel analysis.

To regulate the expression of connexins in cells, plasmids encoding a transactivator and/or a lac-operon IPTG response-dependent Cx43 target gene were transfected into communication-deficient N2a neuroblastoma cells and revealed that expression of Cx 43 in selected clones could be tightly regulated.

Altered gap junctional communication, intercellular signaling, and growth in cultured astrocytes deficient in connexin43

The results suggest that gap junctional inter cellular communication mediated by Cx43 is not critical for astrocyte differentiation but is likely involved in the regulation of intercellular calcium signaling and cell growth.

Gap Junction Channels of Innexins and Connexins: Relations and Computational Perspectives

This review concerns the similarities between Hirudo medicinalis innexins and human connexins from nucleotide and protein sequence level perspectives and sets forth evidence of computational techniques applied to the study of proteins, sequences, and molecular dynamics.

Downregulation of connexin 45 gene products during mouse heart development.

Cx45 gene is the first Cx gene so far demonstrated to be activated in heart at the stage of the first contractions, and the coordination of myocytes during the slow peristaltic contractions that occur at this stage would thus appear to be controlled by the Cx45 channels.

Connexin 30 is expressed in the mouse sino-atrial node and modulates heart rate.

Cx30 is functionally expressed, in low abundance, in the SA node of the adult mouse heart where it participates in HR regulation.

Expression pattern of connexin gene products at the early developmental stages of the mouse cardiovascular system.

During the early stages of mouse heart maturation, between 8.5 days post coitum (dpc), when the first rhythmic contractions appear, and 14.5 dpc, there is clearly a temporary and asymmetrical regionalization of the Cx40 gene expression that is superimposed on the functional regionalization.

In Vivo and In Vitro Expression of Connexin 43 in Human Teeth

Examination of the distribution of connexin 43 in embryonic and permanent intact and carious human teeth suggests that Cx43 expression is developmentally regulated in human dental tissues, and suggests that it may participate in the processes of dentin formation and pathology.

Connexins in mammalian heart function

  • D. GrosH. Jongsma
  • Biology
    BioEssays : news and reviews in molecular, cellular and developmental biology
  • 1996
Recent data dealing with the topographical heterogeneity of expression of these connexins in the different cardiac tissues and the unique conductance properties of the channels they form are reviewed.

Connexin 43 and ischemic preconditioning.



Inhibition of gap junction and adherens junction assembly by connexin and A-CAM antibodies

Functional interactions of the extracellular domains of the connexins were necessary for the formation of gap junction channels and cell interactions mediated by A-CAM were required for gap junction assembly.

Biochemical and immunochemical analysis of the arrangement of connexin43 in rat heart gap junction membranes.

The results unequivocally confirm models of the organization of Cx43 that were based on a more limited set of data and a priori considerations of the sequence.

Reduction of gap junctional conductance by microinjection of antibodies against the 27-kDa liver gap junction polypeptide.

It is shown that immunologically similar polypeptides comprise gap junctions in adult mammalian cells derived from all three germ layers and should provide a tool for further investigation of the role of cell-cell communication mediated by gap junications.

Membrane topology and quaternary structure of cardiac gap junction ion channels.

Restricted distribution of connexin40, a gap junctional protein, in mammalian heart.

The results of immunoelectron microscopy carried out with guinea pig atrial tissue showed that epitopes recognized by these antibodies were exclusively associated with gap junctions, and Cx40 is the first connexin demonstrated in this region of the rat conduction system.

Antisera directed against connexin43 peptides react with a 43-kD protein localized to gap junctions in myocardium and other tissues

A topological model of connexins with unique cytoplasmic domains but conserved transmembrane and extracellular regions is supported, supported by direct evidence that connexin43 is a cardiac gap junction protein and raised rabbit antisera directed against synthetic oligopeptides corresponding to two unique regions of its sequence.

The 43-kD polypeptide of heart gap junctions: immunolocalization, topology, and functional domains

Experimental evidence indicates that, in spite of the differences in amino acid sequence, the gap junction proteins in heart and liver share a general organizational plan and that there may be several domains of the molecule that are involved in the control of junctional permeability.

Topology of the 32‐kd liver gap junction protein determined by site‐directed antibody localizations.

Results indicate a transmembrane orientation for the protein with the amino and carboxyl termini located on the cytoplasmic side of the membrane and a model is proposed for the trans Membrane folding of the gap junction protein.

Evidence that heart connexin43 is a phosphoprotein.