Effect of 5-fluoro-2'-deoxyuridine on deoxyribonucleotide pools in vivo.

@article{Ritter1980EffectO5,
  title={Effect of 5-fluoro-2'-deoxyuridine on deoxyribonucleotide pools in vivo.},
  author={E. J. Ritter and William J. Scott and J. Gerald Wilson and Beatrice C. Lampkin and John E. Neely},
  journal={Journal of the National Cancer Institute},
  year={1980},
  volume={65 3},
  pages={
          603-5
        }
}
5-Fluoro-2'-deoxyuridine (FdUrd) lowered the dTTP levels in rapidly frozen 12-day W rat embryos and in a human neuroblastoma grown in nude N:NIH(S) mice to about 20% of control values. This effect was associated with greatly increased dCTP levels and reduction of dGTP levels essentially to zero. Elimination of the dGTP pool correlated temporally with the cytotoxicity of FdUrd. Extremely rapid fixation of tissue was required to avoid artifactually high deoxyribonucleoside triphosphate values. 
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Nucleotide Pool Imbalance
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NTP and dNTP have become a fundamental tenet for the design of chemotherapeutic agents in the past half century and are maintained by the tightly regulated biosynthetic and metabolic pathways through a number of feedback controls.
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