Effect of 5-aza-2'-deoxycytidine on methylation of the putative imprinted control region of H19 during the in vitro development of vitrified bovine two-cell embryos.

@article{Zhao2012EffectO5,
  title={Effect of 5-aza-2'-deoxycytidine on methylation of the putative imprinted control region of H19 during the in vitro development of vitrified bovine two-cell embryos.},
  author={X M Zhao and Jing-Jing Ren and Wenling Du and Hai-Sheng Hao and Dong Wang and Yan Liu and Tong Qin and Hua-Bin Zhu},
  journal={Fertility and sterility},
  year={2012},
  volume={98 1},
  pages={
          222-7
        }
}
OBJECTIVE To determine the effect of vitrification and 5-aza-2'-deoxycytidine (5-aza-dC) on the methylation levels of the putative imprinted control region (ICR) of H19 and H19 expression in bovine two-cell embryos and their derived blastocysts. DESIGN Experimental animal study. SETTING Academic institution. ANIMAL(S) Abattoir-derived bovine ovaries. INTERVENTION(S) Vitrified two-cell embryos were cultured in vitro to blastocysts with 0.01 μM 5-aza-dC (5-aza-dC group) or without 5-aza… CONTINUE READING