Effect of 40Gy irradiation on the ultrastructure, biochemistry, morphology and cytology during spermatogenesis in the southern green stink bug Nezara viridula (Hemiptera: Pentatomidae)

Abstract

A study on the Nezara viridula male gonad cells was undertaken to compare normal development and development of insect cells after irradiation of 4 instar nymphs with a dose of 40 Gray. Visually, morphologically, biochemically and cytologically the insects were not all uniformly affected by the radiation. In all aspects of development there was a range from severely affected to nearly normal. Irradiated insects appeared to move slowly and were unable to mate with non-irradiated females. The testes of the males varied from bright orange to grey in colour and all were smaller in size than non-irradiated testes. The ultrastructure of the developing sperm showed abnormalities the axonemes, the mitochrondrial derivatives, nebenkern and centrioles. Cytochemically, the main difference observed was the presence of . CC-BY-NC-ND 4.0 International license peer-reviewed) is the author/funder. It is made available under a The copyright holder for this preprint (which was not . http://dx.doi.org/10.1101/171991 doi: bioRxiv preprint first posted online Aug. 3, 2017;

Cite this paper

@inproceedings{Grant2017EffectO4, title={Effect of 40Gy irradiation on the ultrastructure, biochemistry, morphology and cytology during spermatogenesis in the southern green stink bug Nezara viridula (Hemiptera: Pentatomidae)}, author={Pat Grant and J.E and Laban}, year={2017} }