Effect of 1,25(OH)2D3 on its receptor mRNA levels and osteocalcin synthesis in human osteosarcoma cells.

@article{Mahonen1990EffectO1,
  title={Effect of 1,25(OH)2D3 on its receptor mRNA levels and osteocalcin synthesis in human osteosarcoma cells.},
  author={Anitta Mahonen and Asta Pirskanen and Riitta Kein{\"a}nen and Pekka H. M{\"a}enp{\"a}{\"a}},
  journal={Biochimica et biophysica acta},
  year={1990},
  volume={1048 1},
  pages={30-7}
}
Hormone-dependent accumulation of specific binding sites for 1,25(OH)2D3 and changes in human 1,25-dihydroxy-vitamin D receptor (hVDR) mRNA levels were examined in cell lines (MG-63, SaOs-2 and U2-Os) derived from human bone. Osteocalcin synthesis and secretion as well as alkaline phosphatase activity were also characterized as biochemical markers of the osteoblastic phenotype. Specific binding sites for 1,25(OH)2D3 were quantified by incubating cultured intact cells with [3H]1,25(OH)2D3 at 37… CONTINUE READING
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