Effect of 1,24R‐dihydroxyvitamin D3 on the Growth of Human Keratinocytes

@article{Matsumoto1990EffectO1,
  title={Effect of 1,24R‐dihydroxyvitamin D3 on the Growth of Human Keratinocytes},
  author={K. Matsumoto and Koji Hashimoto and Mamoru Kiyoki and M. Yamamoto and Kunihiko Yoshikawa},
  journal={The Journal of Dermatology},
  year={1990},
  volume={17}
}
The effect of 1,24R‐dihydroxyvitamin D3 (1,24R(OH)2D3), a synthetic analogue of a biologically active form of vitamin D3 (1,25‐dihydroxyvitamin D3, 1,25(OH)2D3), on the growth of human keratinocytes cultured in serum‐free medium was investigated. The growth of cultured normal human keratinocytes was inhibited by 65% by 10−8M 1,24R(OH)2D3 and by 90% by 10−7M 1,24(OH)2D3. It inhibited cell growth almost completely at 10−6M. The DNA synthesis of keratinocytes was also inhibited with 1,24R(OH)2D3… 
1α,24R‐Dihydroxyvitamin D3 Has an Ability Comparable to That of 1α,25‐Dihydroxyvitamin D3 to Induce Keratinocyte Differentiation
TLDR
1,24R(OH)2D3 has an ability comparable to that of 1,25(OH), a synthetic derivative of vitamin D3, to induce cell differentiation in human keratinocytes, which indicates that the clinical effectiveness of 2,000 mg/kg D3 for the treatment of psoriasis may be, in part, related to its direct effect on hyperproliferative keratin cells.
The effect of 1,25(OH)2-vitamin D3 on Langerhans cells and contact hypersensitivity in mice
TLDR
The results suggest that 1,25 (OH)z-vitamin D 3 may also have effects on the immune system as an immunoregulatory hormone [8], and langerhans cells (LCs), located in the epidermis of the skin, play an important role in skin immunity.
Vitamin D3 and skin diseases
  • K. Kragballe
  • Biology, Medicine
    Archives of Dermatological Research
  • 2004
TLDR
Because some of the novel vitamin D analogues are potent regulators of cell growth and immune responses, they may be of potential interest in the treatment of ichthyoses, cancer, and autoimmune diseases.
Treatment of psoriasis with calcipotriol and other vitamin D analogues.
  • K. Kragballe
  • Biology, Medicine
    Journal of the American Academy of Dermatology
  • 1992
1α,25-Dihydroxyvitamin-D3-3-bromoacetate regulates AKT/mTOR signaling cascades: a therapeutic agent for psoriasis.
TLDR
BE was significantly more potent than an equivalent concentration of Vit-D in inhibiting growth and survival of human keratinocytes and the antimitotic effect was found to be due to the inhibition of phosphorylation of serine/threonine protein kinase (AKT) and its downstream target, mammalian target of rapamycin (mTOR).
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References

SHOWING 1-10 OF 18 REFERENCES
Effect of 1α,25-dihydroxyvitamin D3 on the morphologic and biochemical differentiation of cultured human epidermal keratinocytes grown in serum-free conditions
TLDR
It appears that 1α,25-(OH) 2 -D 3 is a potent inhibitor of keratinocyte proliferation as well as a stimulator of epidermal terminal differentiation.
Nuclear uptake of 1,25-dihydroxy[3H]cholecalciferol in dispersed fibroblasts cultured from normal human skin.
  • C. Eil, S. Marx
  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1981
TLDR
By using fibroblasts cultured from normal human skin, a process of nuclear uptake of 1,25(OH)2[3H]D3 with the affinity, saturability, and specificity characteristics of a steroid hormone--receptor interaction is identified.
Resistance to 1,25-dihydroxyvitamin D3 of cultured psoriatic epidermal keratinocytes isolated from involved and uninvolved skin.
TLDR
Epidermal keratinocytes isolated from both involved and uninvolved skin of patients with psoriasis were resistant, and 100-fold more 1,25-(OH)2D3 was required to inhibit their DNA synthesis.
Interaction of 1,25-dihydroxyvitamin-D3 with keratinocytes and fibroblasts from skin of normal subjects and a subject with vitamin-D-dependent rickets, type II: a model for study of the mode of action of 1,25-dihydroxyvitamin D3.
TLDR
Multiple analyses by sucrose density gradient or saturation binding assays failed to reveal specific saturable binding of vitamin D-dependent rickets, type II, an inheritable disorder characterized by extreme end-organ resistance to the action of 1,25-(OH)2D3.
Regulation of terminal differentiation of cultured mouse epidermal cells by 1 alpha,25-dihydroxyvitamin D3.
TLDR
Of several derivatives of vitamin D3 examined, 1 alpha,25(OH)2D3 was the most potent in inducing epidermal differentiation and markedly stimulated formation of a cornified envelope, a structure with chemically stable cross-links formed beneath the plasma membrane.
Demonstration of 1,25-dihydroxyvitamin D3 receptors in human skin biopsies.
TLDR
The findings that receptors can be measured in cultured human skin after several arterial passages indicates that skin biopsy may provide a means of assessing the 1,25-(OH)2D3 receptor status of patients.
Cultured psoriatic fibroblasts from involved and uninvolved sites have a partial but not absolute resistance to the proliferation-inhibition activity of 1,25-dihydroxyvitamin D3.
TLDR
C cultured psoriatic fibroblasts from involved and uninvolved skin have a partial resistance to 1,25-(OH)2-D3, suggesting that there may be a biochemical defect that is inherent in the dermal fibro Blasts of psoriasis patients.
Characterization of a receptor-like protein for 1,25-dihydroxyvitamin D3 in rat skin.
  • R. Simpson, H. DeLuca
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1980
TLDR
The presence of a 1,25-dihydroxyvitamin D3 binding macromolecule with properties similar to those of the bone and intestinal receptor suggests that skin is an additional target organ for this hormone.
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