Effect of α-particle and X-ray irradiation on DNA synthesis in tissue cultures

  title={Effect of $\alpha$-particle and X-ray irradiation on DNA synthesis in tissue cultures},
  author={C. Smith},
  journal={Proceedings of the Royal Society of London. Series B. Biological Sciences},
  pages={557 - 570}
  • C. Smith
  • Published 15 August 1961
  • Biology
  • Proceedings of the Royal Society of London. Series B. Biological Sciences
The effects of both α-particle and X-ray in radiation, on the DNA synthesis rate in mouse fibroblast and Hela cells in tissue culture is described. Tritiated thymidine autoradiography was used to indicate the rate of DNA synthesis in the single-layer cultures used. The results of these experiments show that: (1) The fraction of cells in a culture synthesizing DNA is not markedly affected by α-particles and X-rays in the dose used in the experiment. (2) The effect of either type of radiation is… Expand
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