Efeito de diferentes concentrações de melatonina em espermatozoides de carneiros sobre estresse oxidativo após criopreservação

@article{Souza2016EfeitoDD,
  title={Efeito de diferentes concentraç{\~o}es de melatonina em espermatozoides de carneiros sobre estresse oxidativo ap{\'o}s criopreservaç{\~a}o},
  author={W. L. Souza and E. A. Moraes and J. M. S. Costa and P. H. F. Sousa and E. S. L. J{\'u}nior and R. P. Oliveira and R. Toniolli},
  journal={Pesquisa Veterinaria Brasileira},
  year={2016},
  volume={36},
  pages={657-664}
}
The aim was to evaluate the effect of adding different concentrations of melatonin in ram semen diluted after cryopreservation. Ten ejaculates were collected0 from three adult ram (n=30) by means of artificial vagina for sheep. The collected samples were diluted in Tris-egg yolk, to a final concentration 200x106 sptz/mL kept in water bath at 32°C, and melatonin added as treatments: control; 100pM; 100nM; 100μM and 1mM melatonin. Then, the samples were cooled in a cold chamber at 5°C for two… Expand

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