Efavirenz in plasma from HIV-infected patients does not directly block reverse transcriptase activity in cell-free assays but inhibits HIV replication in cellular assays.

@article{Burrer2006EfavirenzIP,
  title={Efavirenz in plasma from HIV-infected patients does not directly block reverse transcriptase activity in cell-free assays but inhibits HIV replication in cellular assays.},
  author={Renaud Burrer and Gabriella Spiridon and Sandrine Einius-Haessig and Sophie Richert and D Salmon-Ceron and Gianfranco Pancino and A. M. Aubertin and Christiane Moog},
  journal={AIDS research and human retroviruses},
  year={2006},
  volume={22 9},
  pages={
          865-9
        }
}
The aim of this study was to characterize the potent nonimmunoglobulin (Ig) inhibitory activity defected in plasma from some HIV-infected, efavirenz (EFV)-treated patients. Concentration of EFV in plasma was measured by HPLC and correlation with reverse transcriptase (RT) inhibition or decrease in virus replication in cellular assays was searched. After plasma protein elimination by ethanol extraction, an inhibitory activity is measurable on RT in vitro that correlates with EFV concentration… CONTINUE READING