Editing of the Bacillus subtilis Genome by the CRISPR-Cas9 System.

Abstract

UNLABELLED The clustered regularly interspaced short palindromic repeat (CRISPR)-associated (Cas) systems are adaptive immune systems of bacteria. A type II CRISPR-Cas9 system from Streptococcus pyogenes has recently been developed into a genome engineering tool for prokaryotes and eukaryotes. Here, we present a single-plasmid system which allows efficient… (More)
DOI: 10.1128/AEM.01453-16

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