Editing DNA Methylation in the Mammalian Genome

  title={Editing DNA Methylation in the Mammalian Genome},
  author={Xiaohong Liu and Hao Wu and Xiong Ji and Yonatan Stelzer and Xuebing Wu and S. Scheller . M. Czauderna and Jian Shu and Daniel B. Dadon and Richard A. Young and Rudolf Jaenisch},
Mammalian DNA methylation is a critical epigenetic mechanism orchestrating gene expression networks in many biological processes. However, investigation of the functions of specific methylation events remains challenging. Here, we demonstrate that fusion of Tet1 or Dnmt3a with a catalytically inactive Cas9 (dCas9) enables targeted DNA methylation editing. Targeting of the dCas9-Tet1 or -Dnmt3a fusion protein to methylated or unmethylated promoter sequences caused activation or silencing… CONTINUE READING