Ectopic expression of genes during chicken limb pattern formation using replication defective retroviral vectors

@article{Pompa1993EctopicEO,
  title={Ectopic expression of genes during chicken limb pattern formation using replication defective retroviral vectors},
  author={Jos{\'e}Luis de la Pompa and Rolf Zeller},
  journal={Mechanisms of Development},
  year={1993},
  volume={43},
  pages={187-198}
}
A gene transfer method to ectopically express genes during chicken limb pattern formation using replication defective retroviral vectors has been established. Spherical non-proliferating (mitomycin C treated) aggregates of clonal retrovirus producing cells were grafted directly into developing chicken wing buds. The cell aggregates had to be placed in direct contact with the highly proliferative cells of the wing bud to promote efficient in vivo infection of embryonic cells by the released… CONTINUE READING