Ecto-alkaline phosphatase considered as levamisole-sensitive phosphohydrolase at physiological pH range during mineralization in cultured fetal calvaria cells.

@article{Anagnostou1996EctoalkalinePC,
  title={Ecto-alkaline phosphatase considered as levamisole-sensitive phosphohydrolase at physiological pH range during mineralization in cultured fetal calvaria cells.},
  author={Fani Anagnostou and Christian Plas and Nadine Forest},
  journal={Journal of cellular biochemistry},
  year={1996},
  volume={60 4},
  pages={
          484-94
        }
}
Alkaline phosphatase (ALP) activity expressed on the external surface of cultured fetal rat calvaria cells and its relationship with mineral deposition were investigated under pH physiological conditions. After replacement of culture medium by assay buffer and addition of p-nitrophenyl phosphate (pNPP), the rate of substrate hydrolysis catalyzed by whole cells remained constant for up to seven successive incubations of 10 min and was optimal over the pH range 7.6-8.2. It was decreased by… CONTINUE READING

Similar Papers

Loading similar papers…