Ecosystem biomonitoring with eDNA: metabarcoding across the tree of life in a tropical marine environment

@article{Stat2017EcosystemBW,
  title={Ecosystem biomonitoring with eDNA: metabarcoding across the tree of life in a tropical marine environment},
  author={M. Stat and Megan J Huggett and Rachele Bernasconi and J. DiBattista and Tina E Berry and S. Newman and E. Harvey and M. Bunce},
  journal={Scientific Reports},
  year={2017},
  volume={7}
}
Effective marine management requires comprehensive data on the status of marine biodiversity. [...] Key Result Directly sequencing eDNA from seawater using a shotgun approach resulted in only 0.34% of 22.3 million reads assigning to eukaryotes, highlighting the inefficiency of this method for assessing eukaryotic diversity. In contrast, using ‘tree of life’ (ToL) metabarcoding and 20-fold fewer sequencing reads, we could detect 287 families across the major divisions of eukaryotes.Expand

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