Economical and Rapid Method for Extracting Cotton Genomic DNA

Abstract

A fast, simple, and reliable mini-prep method for the extraction of DNA from Gossypium species and cultivars has been developed. This small-scale method is cetyltrimethylammonium bromide (cTAB)-based, and it extracts DNA from one to three folded or nearly unfolded young leaves processed in a 1.5 mL tube with 0.5 mL of extraction buffer and homogenized by an… (More)

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