Ecdysone Metabolism in Diapause Eggs and Non-Diapause Eggs of the Silkworm, Bombyx mori.

Abstract

In order to compare ecdysone metabolism between diapause eggs and non-diapause eggs of the silkworm, Bombyx mori, (3)H-ecdysone and its derivatives ((3)H-3-epiecdysone and (3)H-ecdysone 22-phosphate) were injected into the eggs at various stages during early embryogenesis, and the resultant labelled metabolites were analyzed by high-performance liquid chromatography. From the quantitative and qualitative changes in the labelled metabolites between diapause eggs and non-diapause eggs, it was demonstrated that epimerization of ecdysone occurred during early embryogenesis irrespective of the embryonic stage in both diapause eggs and non-diapause eggs, and that phosphorylation of ecdysone was a major metabolic step in diapause eggs, whereas dephosphorylation of ecdysone 22-phosphate and its subsequent hydroxylation at the C-20 and C-26 positions were characteristic in non-diapause eggs.

DOI: 10.2108/zsj.17.89

Cite this paper

@article{Makka2000EcdysoneMI, title={Ecdysone Metabolism in Diapause Eggs and Non-Diapause Eggs of the Silkworm, Bombyx mori.}, author={Toshihiro Makka and Haruyuki Sonobe}, journal={Zoological science}, year={2000}, volume={17 1}, pages={89-95} }