Ebp1, an ErbB‐3 binding protein, interacts with Rb and affects Rb transcriptional regulation

@article{Xia2001Ebp1AE,
  title={Ebp1, an ErbB‐3 binding protein, interacts with Rb and affects Rb transcriptional regulation},
  author={Xianmin Xia and Aiwu Cheng and Tracy J. Lessor and Yue Zhang and Anne W. Hamburger},
  journal={Journal of Cellular Physiology},
  year={2001},
  volume={187}
}
Ebp1, an ErbB‐3 binding protein, inhibits the proliferation and induces the differentiation of human breast cancer cells. The mechanisms of these effects are unknown. Rb, the product of the retinoblastoma gene, is an important modulator of cell cycle progression and cellular differentiation. We report that Rb is a binding target for Ebp1. Ebp1 was localized to both the nucleus and the cytoplasm of logarithmically growing AU565 breast cancer cells and HeLa cells as determined by confocal… Expand
Repression of E2F1-mediated transcription by the ErbB3 binding protein Ebp1 involves histone deacetylases.
TLDR
It is shown here that Ebp1 can inhibit the transcription of other E2F-regulated reporter genes and of several endogenous E2f-regulated genes important in cell cycle progression in both Rb positive and Rb null cells. Expand
Phosphorylation of the ErbB3 binding protein Ebp1 by p21-activated kinase 1 in breast cancer cells
TLDR
It is demonstrated for the first time that Ebp1 is a substrate of PAK1 and the importance of thePAK1 phosphorylation site for the functional activity of Ebp 1 in breast cancer cells is demonstrated. Expand
The ErbB3 binding protein Ebp1 interacts with Sin3A to repress E2F1 and AR-mediated transcription
TLDR
Results demonstrate that Ebp1 participates in transcriptional regulation via its interaction with the Sin3–HDAC and Chromatin immunoprecipitation and DNA affinity precipitation analysis demonstrated that Eb p1 and Sin3A associate at the PSA and E2F1 promoters. Expand
Specificity and heregulin regulation of Ebp1 (ErbB3 binding protein 1) mediated repression of androgen receptor signalling
TLDR
It is suggested that Ebp1 is an AR corepressor whose biological activity can be regulated by the ErbB3/4 ligand, HRG, and this study demonstrates that Eb p1 repressed transcriptional activation of wild-type AR in prostate cancer cell lines. Expand
Heregulin Regulates the Ability of the ErbB3-binding Protein Ebp1 to Bind E2F Promoter Elements and Repress E2F-mediated Transcription*
TLDR
These findings suggest that Ebp1, by linking HRG activation of membrane receptors to E2F gene activity, may be a downstream modulator of the effects of HRG on cell cycle progression. Expand
Ebp1-mediated inhibition of cell growth requires serine 363 phosphorylation.
TLDR
It is shown that serine 363 (S363) of Ebp1 is phosphorylated in vivo, and the importance of S363 phosphorylation in the function of Eb p1 is indicated. Expand
The ErbB3-binding protein Ebp1 suppresses androgen receptor-mediated gene transcription and tumorigenesis of prostate cancer cells.
TLDR
Ebp1 is a previously unrecognized therapeutic target for treatment of hormone refractory prostate cancer and down-regulates expression of AR and AR-regulated genes in the LNCaP prostate cancer cell line. Expand
Repression of androgen receptor mediated transcription by the ErbB-3 binding protein, Ebp1
TLDR
Ebp1 may play a role in the function of the AR and provide a link between ErbB receptors and the AR, and this binding was increased by androgen treatment. Expand
Opposing roles of the two isoforms of ErbB3 binding protein 1 in human cancer cells
TLDR
The aim of the current review is to provide a summary on distinctive cellular functions of two Ebp1 proteins and their molecular partners that might be responsible for the unique functions of each isoform of Ebp 1. Expand
Inhibition of heregulin mediated MCF-7 breast cancer cell growth by the ErbB3 binding protein EBP1.
TLDR
Down-regulation of EBP1 expression in MCF-7 cells by shRNA resulted in increased cell growth in response to HRG and increased cyclin D1 and ErbB2 expression, suggesting that EBP 1, by down-regulating Erb B signal transduction, attentuates HRG-mediated growth of breast cancer cells. Expand
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TLDR
It is demonstrated that ectopic expression of the ErbB‐3 binding protein Ebp1 inhibits proliferation and induces differentiation of ErBB‐2, Erb B‐3–expressing human breast carcinoma cell lines. Expand
RB and hbrm cooperate to repress the activation functions of E2F1.
TLDR
It is demonstrated that the domain of hbrm that binds RB has transcriptional activation potential which RB can repress, suggesting that RB not only targets hBRm but also regulates its activity. Expand
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TLDR
Using the yeast two-hybrid system, an ErbB-3 binding protein (Ebp1) is isolated that interacts with the juxtamembrane domain of Erb B-3 and is identical to that predicted to be encoded for by the human PA2G4 gene. Expand
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TLDR
The results suggest that HBP1 may represent a unique transcriptional repressor with a role in initiation and establishment of cell cycle arrest during differentiation. Expand
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TLDR
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Rb and Prohibitin Target Distinct Regions of E2F1 for Repression and Respond to Different Upstream Signals
TLDR
It is demonstrated that prohibitin requires the marked box region of E2F for repression; further, prohibitin can effectively inhibit colony formation induced by overexpression of E 2F1 in T47D cells. Expand
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TLDR
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TLDR
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TLDR
It is shown here that RBP1, a known pRB pocket-binding protein, possesses transcriptional repression activity and associates with p130-E2F and pRB-E1F complexes specifically during growth arrest and may play a central role in inducing growth arrest. Expand
Stable binding to E 2 F is not required for the retinoblastoma protein to activate transcription , promote differentiation , and suppress tumor cell growth
The retinoblastoma tumor suppressor protein (pRB) can inhibit cell cycle progression and promote differentiation. pRB interacts with a variety of transcription factors, including members of the E2FExpand
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