Easy preparation of a large-size random gene mutagenesis library in Escherichia coli.

@article{You2012EasyPO,
  title={Easy preparation of a large-size random gene mutagenesis library in Escherichia coli.},
  author={Chun Xue You and Y-H Percival Zhang},
  journal={Analytical biochemistry},
  year={2012},
  volume={428 1},
  pages={7-12}
}
A simple and fast protocol for the preparation of a large-size mutant library for directed evolution in Escherichia coli was developed based on the DNA multimers generated by prolonged overlap extension polymerase chain reaction (POE-PCR). This protocol comprised the following: (i) a linear DNA mutant library was generated by error-prone PCR or shuffling… CONTINUE READING