Easy assembly of ligands for glycosidase affinity chromatography.


An improved, high-yield synthesis of the corresponding N-carboxypentyl derivatives of three iminoalditol glycosidase inhibitors has been developed for affinity chromatography enzyme purification. Reductive amination of 1-deoxynojirimycin (or its D-manno or D-galacto analogues) with methyl 5-formylvalerate and NaBH3CN at neutral pH afforted an aminoester… (More)


Cite this paper

@article{Bernotas1990EasyAO, title={Easy assembly of ligands for glycosidase affinity chromatography.}, author={Ronald C Bernotas and B. Ganem}, journal={The Biochemical journal}, year={1990}, volume={270 2}, pages={539-40} }