Easy and Rapid Purification of Highly Active Nisin

Abstract

Nisin is an antimicrobial peptide produced and secreted by several L. lactis strains and is specifically active against Gram-positive bacteria. In previous studies, nisin was purified via cation exchange chromatography at low pH employing a single-step elution using 1 M NaCl. Here, we describe an optimized purification protocol using a five-step NaCl… (More)
DOI: 10.1155/2011/175145

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6 Figures and Tables