Early stages in DNA binding and uptake during genetic transformation of pneumococci.

@article{Seto1974EarlySI,
  title={Early stages in DNA binding and uptake during genetic transformation of pneumococci.},
  author={Haruo Seto and Alexander Tomasz},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1974},
  volume={71 4},
  pages={1493-8}
}
Ethylenediaminetetraacetate and other divalent-cation-complexing agents greatly stimulate the cellular binding of DNA molecules to competent pneumococci, while the appearance of genetic transformants and nuclease-resistant DNA binding are completely inhibited. Based on this finding, we developed an experimental system in which three early and consecutive stages of genetic transformation can be experimentally separated: (i) attachment of DNA molecules to cell surface sites that are only… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.