Early infant human immunodeficiency virus type 1 detection suitable for resource-limited settings with multiple circulating subtypes by use of nested three-monoplex DNA PCR and dried blood spots.

Abstract

The early detection of human immunodeficiency virus type 1 (HIV-1) infection in infants is complicated by the persistence of maternal antibodies and by diverse HIV-1 subtypes. We developed a nested, three-monoplex HIV-1 DNA PCR (N3M-PCR) assay to detect diverse HIV-1 subtypes in infants born to infected mothers. We optimized the test for use with dried… (More)

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Cite this paper

@article{Zhang2008EarlyIH, title={Early infant human immunodeficiency virus type 1 detection suitable for resource-limited settings with multiple circulating subtypes by use of nested three-monoplex DNA PCR and dried blood spots.}, author={Qi Zhang and Limin Wang and Yan Jiang and Liwen Fang and Pinliang Pan and Shuangyan Gong and Jun Yao and Yi-Wei Tang and Sten H Vermund and Yujiang Jia}, journal={Journal of clinical microbiology}, year={2008}, volume={46 2}, pages={721-6} }