Early events in the replication of Mu prophage DNA.

  title={Early events in the replication of Mu prophage DNA.},
  author={Barbara T. Waggoner and Martin Leon Pato},
  journal={Journal of virology},
  volume={27 3},
To determine whether the early replication of Mu prophage DNA proceeds beyond the termini of the prophage into hose DNA, the amounts of both Mu DNA and the prophage-adjacent host DNA sequences were measured using a DNA-DNA annealing assay after induction of the Mu vegetative cycle. Whereas Mu-specific DNA synthesis began 6 to 8 min after induction, no amplification of the adjacent DNA sequences was observed. These data suggest that early Mu-induced DNA synthesis is constrained within the… CONTINUE READING