Early effects of PRL on ion conductances in CHO cells expressing PRL receptor.

@article{Prevarskaya1994EarlyEO,
  title={Early effects of PRL on ion conductances in CHO cells expressing PRL receptor.},
  author={Natacha Prevarskaya and Roman Skryma and Pierre Vacher and Nathalie Daniel and Christophe Bignon and Jean Djiane and Bernard Dufy},
  journal={The American journal of physiology},
  year={1994},
  volume={267 2 Pt 1},
  pages={C554-62}
}
Chinese hamster ovary (CHO-K1) cells were stably transfected with prolactin (PRL) receptor cDNA. These cells (CHO-E32) expressed the long form of functional PRL receptor. Using microfluorimetric and patch-clamp techniques, we have investigated the effects of PRL on intracellular Ca2+ concentration ([Ca2+]i) and membrane ion conductances. Exposure of CHO-E32 cells to 5 nM PRL resulted in an increase in [Ca2+]i. Two types of response were observed: 1) a stimulation of Ca2+ entry and 2) an… CONTINUE READING