Early detection of sugar beet pathogen Ramularia beticola in leaf and air samples using qPCR


A quantitative PCR method (qPCR) was developed for the detection and quantification of Ramularia beticola causing Ramularia leaf spot in sugar beet. R. beticola specific primers were designed based on the internal transcribed spacer region 2 (ITS2). The assay was applied on DNA extracted from spores trapped on tape from Burkard spore traps placed in an… (More)
DOI: 10.1007/s10658-013-0349-6


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