Early detection of bovine leukemia virus in cattle by use of the polymerase chain reaction.

@article{Kelly1993EarlyDO,
  title={Early detection of bovine leukemia virus in cattle by use of the polymerase chain reaction.},
  author={Erica J Kelly and M. K. Jackson and G. Marsolais and John D. Morrey and Robert J. Callan},
  journal={American journal of veterinary research},
  year={1993},
  volume={54 2},
  pages={205-9}
}
A study was performed to determine whether experimentally induced bovine leukemia virus (BLV) infection in cattle could be detected earlier by use of polymerase chain reaction (PCR) amplification of genomic DNA extracted from leukocytes than by use of conventional agar-gel immunodiffusion (AGID). The PCR primers were designed to amplify a 375-base-pair region of the proviral gag gene. Five cows were identified that were BLV-negative on the basis of AGID and PCR results. At day 0, these cows… CONTINUE READING