Early detection of PrPres in BSE-infected bovine PrP transgenic mice

  title={Early detection of PrPres in BSE-infected bovine PrP transgenic mice},
  author={Joaqu{\'i}n Castilla and Ana Adan and Alejandro Brun and Bel{\'e}n Pintado and Miguel A Ram{\'i}rez and Beatriz Parra and Deirdre Doyle and Mark Rogers and Francisco J Salguero and Carmen Sanchez and Jos{\'e} M. S{\'a}nchez-Vizca{\'i}no and Juan Maria Torres},
  journal={Archives of Virology},
 Transgenic mouse lines expressing different levels of the bovine prion protein gene (boPrPC) were generated. Upon infection with BSE prions, all transgenic lines tested exhibited characteristics of the bovine disease. Typical CNS spongiform degeneration was observed by histopathology and presence of PrPres could be detected both by Western blot and immunohistochemistry (IHC) assays, confirming for this model the absence of an interspecies barrier to BSE infection. Differences in incubation… CONTINUE READING