Early detection: The power and the promise of DNA methylation markers

@article{Laird2003EarlyDT,
  title={Early detection: The power and the promise of DNA methylation markers},
  author={Peter W. Laird},
  journal={Nature Reviews Cancer},
  year={2003},
  volume={3},
  pages={253-266}
}
  • P. Laird
  • Published 1 April 2003
  • Biology
  • Nature Reviews Cancer
The past few years have seen an explosion of interest in the epigenetics of cancer. This has been a consequence of both the exciting coalescence of the chromatin and DNA methylation fields, and the realization that DNA methylation changes are involved in human malignancies. The ubiquity of DNA methylation changes has opened the way to a host of innovative diagnostic and therapeutic strategies. Recent advances attest to the great promise of DNA methylation markers as powerful future tools in the… 

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References

SHOWING 1-10 OF 187 REFERENCES

Cancer-epigenetics comes of age

TLDR
Current mechanistic understanding of the role of DNA methylation in malignant transformation is reviewed, and it is suggested Knudson's two–hit hypothesis should be expanded to include epigenetic mechanisms of gene inactivation.

The fundamental role of epigenetic events in cancer

TLDR
This review discusses patterns of DNA methylation and chromatin structure in neoplasia and the molecular alterations that might cause them and/or underlie altered gene expression in cancer.

DNA methylation in health and disease

TLDR
DNA methylation has recently moved to centre stage in the aetiology of human neurodevelopmental syndromes such as the fragile X, ICF and Rett Syndromes, and understanding this link will be central to the design of new therapeutic tools.

Cancer epigenomics.

  • C. Plass
  • Biology
    Human molecular genetics
  • 2002
TLDR
This review will summarize the current knowledge of DNA methylation and histone modification in normal cells, introduce emerging concepts that show the intimate link between DNAmethylation and chromatin modifications, and highlight recent advancements in the understanding of aberrant DNA methylations, with special emphasis on genome-wide hypermethylation.

Oncogenic mechanisms mediated by DNA methylation.

  • P. Laird
  • Biology
    Molecular medicine today
  • 1997

DNA methylation differences associated with tumor tissues identified by genome scanning analysis.

TLDR
Hypermethylation of CpG islands in cancer cells is not confined to the promoters of growth regulatory genes but is also found in actively transcribed regions of genes.

Applications of CpG island microarrays for high-throughput analysis of DNA methylation.

TLDR
The DMH protocol is outlined and its utility and the validation approaches used in analyzing the hypermethylation profile of breast tumor and normal samples are illustrated.

Sensitive Detection of DNA Methylation

TLDR
The sensitive detection of methylation will provide a powerful diagnostic and prognostic tool, especially for the early detection of preneoplastic and neoplastic lesions.

Epigenomics: genome-wide study of methylation phenomena.

TLDR
The current and future potential of epigenetics is reviewed and the pilot study towards the Human Epigenome Project (HEP) is introduced, introducing the new field, epigenomics.

Predicting lung cancer by detecting aberrant promoter methylation in sputum.

TLDR
It is demonstrated that aberrant methylation of the p16 and/or O6-methyl-guanine-DNA methyltransferase promoters can be detected in DNA from sputum in 100% of patients with squamous cell lung carcinoma up to 3 years before clinical diagnosis.
...