Early changes in the binding between DNA and histone in human leucocytes exposed to phytohemagglutinin.

@article{Zetterberg1969EarlyCI,
  title={Early changes in the binding between DNA and histone in human leucocytes exposed to phytohemagglutinin.},
  author={A. Zetterberg and G. Auer},
  journal={Experimental cell research},
  year={1969},
  volume={56 1},
  pages={
          122-6
        }
}
  • A. Zetterberg, G. Auer
  • Published 1969
  • Biology, Medicine
  • Experimental cell research
  • Abstract Previously reported changes in the deoxyribonucleoprotein (DNP) complex of human leucocytes responding to phytohemagglutinin (PHA) were further investigated in the present work by using the alkaline bromphenol blue (BPB) binding reaction for basic proteins. The extraction of DNA preceding the BPB-binding was performed both in trichloroacetic acid (TCA) and in picric acid (PA). TCA liberates both arginine and lysine residues for the subsequent BPB-binding. PA, which was found to bind… CONTINUE READING
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