Early Steps in Assembly of the Yeast Vacuolar H+-ATPase*

@article{Kane1999EarlySI,
  title={Early Steps in Assembly of the Yeast Vacuolar H+-ATPase*},
  author={Patricia M. Kane and Maureen Tarsio and Jianzhong Liu},
  journal={The Journal of Biological Chemistry},
  year={1999},
  volume={274},
  pages={17275 - 17283}
}
Vacuolar proton-translocating ATPases are composed of a complex of integral membrane proteins, the Vo sector, attached to a complex of peripheral membrane proteins, the V1 sector. We have examined the early steps in biosynthesis of the yeast vacuolar ATPase by biosynthetically labeling wild-type and mutant cells for varied pulse and chase times and immunoprecipitating fully and partially assembled complexes under nondenaturing conditions. In wild-type cells, several V1 subunits and the 100-kDa… 

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