Early HIV-1 diagnosis using in-house real-time PCR amplification on dried blood spots for infants in remote and resource-limited settings.

@article{NgoGiangHuong2008EarlyHD,
  title={Early HIV-1 diagnosis using in-house real-time PCR amplification on dried blood spots for infants in remote and resource-limited settings.},
  author={Nicole Ngo-Giang-Huong and Woottichai Khamduang and Baptiste Leurent and Intira Jeannie Collins and Issaren Nantasen and Pranee Leechanachai and Wasna Sirirungsi and Aram Limtrakul and Tasana Leusaree and Anne Marie Comeau and Marc J Lallemant and Gonzague Jourdain},
  journal={Journal of acquired immune deficiency syndromes},
  year={2008},
  volume={49 5},
  pages={465-71}
}
BACKGROUND In resource-limited settings, most perinatally HIV-1-infected infants do not receive timely antiretroviral therapy because early HIV-1 diagnosis is not available or affordable. OBJECTIVE To assess the performance of a low-cost in-house real-time polymerase chain reaction (PCR) assay to detect HIV-1 DNA in infant dried blood spots (DBS). METHODS One thousand three hundred nineteen DBS collected throughout Thailand from non-breast-fed infants born to HIV-1-infected mothers were… CONTINUE READING