EST-library construction using spore RNA of the arbuscular mycorrhizal fungus Gigaspora rosea

@article{Stommel2001ESTlibraryCU,
  title={EST-library construction using spore RNA of the arbuscular mycorrhizal fungus Gigaspora rosea},
  author={Michaela Stommel and Petra Mann and Philipp Franken},
  journal={Mycorrhiza},
  year={2001},
  volume={10},
  pages={281-285}
}
RNA was extracted from activated spores of the arbuscular mycorrhizal fungus Gigaspora rosea. Double-stranded cDNA was synthesised, digested and cloned into the vector lambda-ZAP express. Of the 1,500 clones obtained, 1.5% carried inserts of the rRNA gene cluster. After excision, inserts from 50 randomly selected clones were sequenced. Database searches revealed that 62% of the clones had similarities to already known sequences. These mainly code for proteins involved in translation and protein… CONTINUE READING
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