EST-derived microsatellites from Actinidia species and their potential for mapping

@article{Fraser2003ESTderivedMF,
  title={EST-derived microsatellites from Actinidia species and their potential for mapping},
  author={Lena G. Fraser and Catherine F Harvey and Ross N. Crowhurst and Harindra de Silva},
  journal={Theoretical and Applied Genetics},
  year={2003},
  volume={108},
  pages={1010-1016}
}
To increase the speed and reduce the cost of constructing a genetic map of Actinidia species (kiwifruit), for use in both breeding and functional genomics programmes, we sampled microsatellites from expressed sequence tags (ESTs) to evaluate their frequency of occurrence and level of polymorphism. Perfect dinucleotide repeats were the microsatellites selected, and these were found to be numerous in both the 5′ and 3′ ends of the genes represented. The microsatellites were of various lengths… CONTINUE READING
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